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Monoclonal Anti-HA antibody produced in mouse
Monoclonal Anti-HA antibody produced in mouse from MilliporeSigma
Be the first to write a review!
Citations:
(879)
Supplier Page
Supplier Page from
MilliporeSigma for
Monoclonal Anti-HA antibody produced in mouse
MilliporeSigma's
Monoclonal Anti-HA antibody produced in mouse
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blot.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Monoclonal Anti-HA antibody produced in mouse
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
H9658
Quantity
0.2ML, 100µL
Applications
indirect ELISA, western blot, immunocytochemistry, immunoprecipitation (IP)
Conjugate/Tag
Unconjugated
Target
HA
Isotype
IgG1
Clone
HA-7
Host
mouse
Clonality
Monoclonal
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (879)
(1)
Active DHEA uptake in the prostate gland correlates with aggressive prostate cancer
J Clin Invest
December 15, 2023
Xuebin Zhang, Zengming Wang, Shengsong Huang, Dongyin He, Weiwei Yan, Qian Zhuang, Zixian Wang, Chenyang Wang, Qilong Tan, Ziqun Liu,
et al.
(anti-GST) (
Cell Signaling Technology
,
2624
), anti-MYC (
Millipore
,
06-549
), anti-FLAG (
Sigma-Aldrich
,
H9658
), and anti–β-actin (
ABclonal
, China,
AC026
). For stable cell lines, HEK293T cells were
transfected
with
(2)
H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome
Nature Communications
November 18, 2023
Zhen Wang, Claudia M. Castillo-González, Changjiang Zhao, Chun-Yip Tong, Changhao Li, Songxiao Zhong, Zhiyang Liu, Kaili Xie, Jiaying Zhu, Zhongshou Wu,
et al.
first incubated with antibodies against Myc (
Sigma
,
C3956
), H3 (
Agrisera
,
AS10 710
), or HA (
Sigma
,
H9658
) and then incubated with
goat
-developed anti-rabbit (GE Healthcare, NA934) or
goat
-developed anti-mouse
(3)
MLKL regulates Cx43 ubiquitinational degradation and mediates neuronal necroptosis in ipsilateral thalamus after focal cortical infarction
Molecular Brain
October 30, 2023
Yanyan Tang, Quanhong Chu, Guanfeng Xie, Yafu Tan, Ziming Ye, Chao Qin
anti-V5-Tag (Cell Signaling Technology Cat# 13,202, RRID:AB_2687461), mouse anti-HA-Tag (
Sigma-Aldrich
Cat#
H9658
, RRID:AB_260092), anti-Myc-Tag (
Cell Signaling Technology
Cat#
2278
, RRID:AB_490778), and anti-
GAPDH
(4)
SARS-COV-2 protein NSP9 promotes cytokine production by targeting TBK1
Frontiers in Immunology
October 2, 2023
Yihua Zhang, Bowen Xin, Yinan Liu, Wenyi Jiang, Wendong Han, Jian Deng, Peihui Wang, Xiaowu Hong, Dapeng Yan
anti-phospho- SAPK/
JNK
(
4668
; all from
Cell Signaling Technology
); anti-Flag M2 Affinity Gel (
A2220
), anti-HA (
H9658
), anti-HA (
H6908
), and anti-Flag (
F7425
; all from
Sigma-Aldrich
); anti-
GST
(CW0084M) and anti-His (CW0286;
(5)
Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication
Virologica Sinica
October 1, 2023
Linlin Zhang, Yali Duan, Wei Wang, Qi Li, Jiao Tian, Yun Zhu, Ran Wang, Zhengde Xie
anti-LC3 antibody (
L7543
), rabbit anti-GFP tag antibody (
SAB4301138
), and mouse anti-HA tag antibody (
H9658
) were purchased from
Sigma-Aldrich
(St. Louis, Missouri, USA). Rabbit anti-SQSTM1/p62 antibody (
5114S
),
(6)
Annexin A2 Stabilizes Oncogenic JAG1 Intracellular Domain by Inhibiting Proteasomal Degradation in Glioblastoma Cells
International Journal of Molecular Sciences
September 30, 2023
Seok Won Ham, Jung Yun Kim, Sunyoung Seo, Nayoung Hong, Min Ji Park, Yoonji Kim, Junseok Jang, Sehyeon Park, Silvee Jisoo Lee, Jun-Kyum Kim,
et al.
rabbit IgG isotype control
(
10500C
;
Thermo Fisher Scientific
), HA (
3724
;
Cell Signaling Technology
), HA (
H9658
,
Sigma-Aldrich
), FLAG (
F7425
;
Sigma-Aldrich
), β-ACTIN (
sc-47778
;
Santa Cruz Biotechnology
, Dallas, TX,
(7)
Knockdown of BAP31 Downregulates Galectin-3 to Inhibit the Wnt/β-Catenin Signaling Pathway to Modulate 5-FU Chemosensitivity and Cancer Stemness in Colorectal Cancer
International Journal of Molecular Sciences
September 21, 2023
Jingjing Liu, Qi Zhang, Jiyu Wang, Changli Wang, Tian Lan, Tianyi Wang, Bing Wang
Antibody,Source,Cat#,Dilution β-actin,
Sigma
,
A1978
,"
1:10,000
" BAP31,
Sigma
,
SAB1406931
,
1:1000
HA,
Sigma
,
H9658
,"
1:10,000
"
Histone H3
,Abcam,ab308373,
1:1000
CD44
,
Abcam
,
ab254530
,
1:2000
CD133
,
Abcam
,
ab222782
,
1:2000
Oct4,
Abcam
,
ab19857
,
1:1000
SOX2,
Abcam
,
ab97959
,
1:1000
Nanog,
Abcam
,
ab21624
,
1:1000
c-MYC
,
Abcam
,
ab185656
,
1:2000
Galectin-3,Abcam,b2785,
1:1000
AKT
,
CST
,
4685S
,
1:1000
p-AKT,
CST
,
4060T
,
1:2000
GSK-3β,
Proteintech
,
67558-1-Ig
,
1:2000
p-GSK-3β,
Proteintech
,
22104-1-AP
,
1:2000
β-catenin,
Proteintech
,
51067-2-AP
,
1:2000
Anti-Mouse
(8)
Prefoldin 2 contributes to mitochondrial morphology and function
BMC Biology
September 12, 2023
Ismail Tahmaz, Somayeh Shahmoradi Ghahe, Monika Stasiak, Kamila P. Liput, Katarzyna Jonak, Ulrike Topf
ADI-SPA-1040D), anti-FLAG tag (catalog no.
F1804
,
Sigma
, diluted
1:1000
), anti-HA tag (catalog no.
H9658
,
Sigma
, diluted
1:5000
), and anti-GFP (catalog no. 118144600001).
Membranes
were incubated with anti-rabbit
(9)
Inferring the Effects of Protein Variants on Protein–Protein Interactions with Interpretable Transformer Representations
Research (Wash D C)
September 11, 2023
Zhe Liu, Wei Qian, Wenxiang Cai, Weichen Song, Weidi Wang, Dhruba Tara Maharjan, Wenhong Cheng, Jue Chen, Han Wang, Dong Xu,
et al.
detected by
immunoblotting
with indicated antibodies. Antibodies used in the study were anti-HA (
Sigma
,
H9658
), anti-SMAD3 (Absin, abs124584), and anti-TP53 (Absin, abs116840). SKEMPI v2 dataset preprocessing SKEMPI
(10)
Sm-like protein Rof inhibits transcription termination factor ρ by binding site obstruction and conformational insulation
bioRxiv
September 7, 2023
Nelly Said, Mark Finazzo, Tarek Hilal, Bing Wang, Tim Luca Selinger, Daniela Gjorgjevikj, Irina Artsimovitch, Markus C. Wahl
Cat.
162-0112
).
Membrane
was incubated with primary anti-HA antibody from
mice
(
Sigma-Aldrich
, Cat.
H9658
) and with secondary anti-
mouse
horseradish
peroxidase linked antibodies (Amersham, Cat. NA931V) before
(11)
MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
September 1, 2023
Fang D, Hu H, Zhao K, Xu A, Yu C, Zhu Y, Yu N, Yao B, Tang S, Wu X,
et al
.
(GFP) (
Santa Cruz
,
sc‐9996
,
1:1000
), Flag (
Proteintech
,
20543‐1‐AP
,
1:4000
), hemagglutinin (HA) (
Sigma
,
H9658
,
1:4000
),
p53
for western blot
(
Santa Cruz
,
sc‐126
,
1:1000
),
p53
for
immunofluorescence
(Santa Cruz,
(12)
Dna2 removes toxic ssDNA-RPA filaments generated from meiotic recombination-associated DNA synthesis
Nucleic Acids Research
August 25, 2023
Binyuan Zhai, Shuxian Zhang, Bo Li, Jiaming Zhang, Xuan Yang, Yingjin Tan, Ying Wang, Taicong Tan, Xiao Yang, Beiyi Chen,
et al.
membranes
(
Millipore
, Cat#
HATF00010
). Primary antibodies used were
mouse
monoclonal anti-HA (
Sigma
, Cat#
H9658
),
mouse
monoclonal anti-
PGK1
(
Abcam
, Cat#
ab113687
), rabbit polyclonal
anti-Rad53
(
Abcam
, Cat#
ab104232
),
(13)
Deep screening of proximal and distal splicing-regulatory elements in a native sequence context
bioRxiv
August 25, 2023
Yocelyn Recinos, Dmytro Ustianenko, Yow-Tyng Yeh, Xiaojian Wang, Martin Jacko, Lekha V. Yesantharao, Qiyang Wu, Chaolin Zhang
0.45 μM nitrocellulose
membrane
(GE Healthcare), the primary antibody mouse anti-HA (
Sigma-Aldrich
,
H9658
,
1:2,000
), and
mouse
anti-alpha-tubulin (
Sigma-Aldrich
,
T6074
,
1:10,000
) were used. After incubating
(14)
The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy
Cell death & disease
August 22, 2023
Pai YL, Lin YJ, Peng WH, Huang LT, Chou HY, Wang CH, Chien CT, Chen GC.
10473-1-AP
), anti-p62 (
MBL
,
PM045
), anti-Flag M2 (
Sigma
,
F1804
), anti-
MYC
(
Santa Cruz
,
SC-40
), anti-HA (
Sigma
,
H9658
), anti-
ACTB
/β-actin (
Novus
,
NB600-501
), anti-tubulin (
Sigma
,
T0198
), anti-GFP (
Abcam
,
ab290
), anti-ubiquitin
(15)
Characterization of the Effects of Host p53 and Fos on Gallid Alpha Herpesvirus 1 Replication
Genes
August 12, 2023
Liu Z, Cui L, Li X, Xu L, Zhang Y, Han Z, Liu S, Li H.
size. Each ChIP experiment used 5 g of either anti-HA (
Sigma-Aldrich
, St. Louis, MO, USA,
mouse
, #
H9658
) or isotype control IgG1 (
Cell Signaling
, Beverly, MA, USA,
mouse
,
5415
) antibodies to shear
chromatin
(16)
ATM-ESCO2-SMC3 axis promotes 53BP1 recruitment in response to DNA damage and safeguards genome integrity by stabilizing cohesin complex
Nucleic acids research
August 11, 2023
Fu J, Zhou S, Xu H, Liao L, Shen H, Du P, Zheng X.
studies:
mouse
monoclonal anti-Flag (
1:10000
,
F3165
,
Sigma-Aldrich
),
mouse
monoclonal anti-HA (
1:10 000
,
H9658
,
Sigma-Aldrich
), rabbit polyclonal anti-53BP1 (IF
1:200
,
sc-22760
,
Santa Cruz Biotechnology
), rabbit
(17)
FAT10 and NUB1L cooperate to activate the 26S proteasome
Life Science Alliance
August 1, 2023
Florian Brockmann, Nicola Catone, Christine Wünsch, Fabian Offensperger, Martin Scheffner, Gunter Schmidtke, Annette Aichem
A-6457. Anti-Flag IgG (order number F-1804) and anti-HA–IgG antibodies were purchased from
Sigma-Aldrich
(
H9658
). Generation of radioactive poly-ubiquitylated
p53
and E6AP For
in vitro
ubiquitylation experiments,
(18)
Functional consequences of C-terminal mutations in RUNX2
Scientific reports
July 27, 2023
Thaweesapphithak S, Theerapanon T, Rattanapornsompong K, Intarak N, Kanpittaya P, Trachoo V, Porntaveetus T, Shotelersuk V.
(PVDF)
membrane
.
Mouse
monoclonal anti-HA antibody (
1:20,000
, Sigma-Aldrich,
Merck
, MO, USA, Cat. No.
H9658
) was used as the primary antibody. The antibody against GAPDH (
1:3000
,
Abcam
, UK, Cat. No.
ab8245
) was
(19)
Spindle Position Checkpoint Kinase Kin4 Regulates Organelle Transport in Saccharomyces cerevisiae
Biomolecules
July 10, 2023
Lakhan Ekal, Abdulaziz M. S. Alqahtani, Maya Schuldiner, Einat Zalckvar, Ewald H. Hettema, Kathryn R. Ayscough
Cambridge, UK 459250). Myo2-HA was detected by a monoclonal anti-HA antibody (
mouse
;
1:5000
;
Sigma-Aldrich
,
H9658
). The secondary antibody was an HRP-linked anti-
mouse
polyclonal (
goat
;
1:4000
;
Bio-Rad
, Watford, UK.
(20)
The HSPB1-p62/SQSTM1 functional complex regulates the unconventional secretion and transcellular spreading of the HD-associated mutant huntingtin protein
Human molecular genetics
July 4, 2023
Bonavita R, Scerra G, Di Martino R, Nuzzo S, Polishchuk E, Di Gennaro M, Williams SV, Caporaso MG, Caiazza C, Polishchuk R,
et al
.
anti-FLAG antibody (
Sigma-Aldrich
, Milan, Italy; #
F1804
);
mouse
monoclonal anti-HA (
Sigma-Aldrich
; #
H9658
,
clone
HA7); rabbit polyclonal anti-LC3 (
Novus Biologicals
, USA; #
NB100-2220
);
mouse
monoclonal anti-LAMP1
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Reviews
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Monoclonal Anti-HA antibody produced in mouse from MilliporeSigma
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